Bio-sketch

Ashok Dubey joined Netaji Subhas Institute of Technology during May 2001 as Professor of Biotechnology. He initiated and led the development and implementation of a four-year Degree Programme in B. E. Biotechnology, under the Faculty of Technology, Delhi University. He was instrumental in establishing the Division of Biotechnology at NSIT and has since been its founding Head of Department.
Professor Dubey was appointed as an Assistant Professor at Indian Institute of Technology-Delhi during 1991 while he was a Post-Doctoral Fellow in the laboratory of Nobel Laureate, Dr. R. J. Roberts at Cold Spring Harbor, New York, USA. Subsequently, he moved from IIT-Delhi to the Biomolecular Research Institute at the Division of Health Sciences of CSIRO in Melbourne, Australia in January, 1998 and continued there until his move to NSIT.
Professor Dubey has worked extensively on restriction enzymes and 5-methyl Cytosine DNA methyltransferase, MspI while working at Cold Spring Harbor and IIT Delhi, which led to several high impact publications. He further studied modulation of foreign gene expression in recombinant Escherichia coli and developed methods of purification and characterization of recombinant proteins including envelope glycoprotein E2 of the Hepatitis C Virus. His main research interests at NSIT include the discovery of anti-microbial agents, novel therapeutics for inflammation, diabetes and cancer; along with further exploring the diversity of actinobacteria.

Areas of Interest

• Anti-bacterial and anti-fungal agents
• Therapeutics for inflammation, diabetes and cancer
• Diversity of actinobacteria and Pesticide Bioremediation

Publications (Peer Reviewed)

1. Singh, R.; Ali, M. and Dubey, A. K. (2024) Identification and characterization of a novel decalin derivative with anti-Candida activity from Streptomyces chrestomyceticus strain ADP4. Arch. Microbiol. https://doi.org/10.1007/s00203-023-03788-2
2. Singh, S.; Dubey, A. K. and Sharma, S. (2023) Sonication-based nanosuspension formation of microbial extract to assess their antibacterial properties. Ind. J Engg. Material Sci. 30(5).671-675. DOI: 10.56042/ijems.v30i5.6853.
3. Singh, R; Ali. M. and Dubey, A. K. (2023) Phenyl pentyl ketone and m-isobutyl methoxy benzoate produced by Streptomyces chrestomyceticus ADP4 are potent antimicrobial agents displaying broad spectrum activities. Ind. J. Microbiol. 63(2): 181-189. DOI: 10.1007/s12088-023-01068-7.
4. Singh, R; Ali. M. and Dubey, A. K. (2023) Anti-Candida attributes and in-silico drug-likeness properties of phenyl 2β, 6β-trimethyl cyclohexyl ketone and phenyl nonanyl ether produced by Streptomyces chrestomyceticus ADP4. J. Appl. Microbiol. 134(1): lxac024. DOI: https://doi.org/10.1093/jambio/lxac024
5. Srivastava, A.; Mishra, S.; Garg, P. K.; Dubey, A. K.; Deo, S. V. S. and Verma, D. (2022) Comparative and analytical characterization of the oral bacteriome of smokeless tobacco users with oral squamous cell carcinoma. Appl. Microbiol. Biotechnol. 106(11): 4115-4128. DOI: https://doi.org/10.1007/s00253-022-11980-5
6. Verma, D., Srivastava, A., Garg, P. K., Akhter, Y., Dubey, A. K., Mishra, S. D. and Deo, S. V. S. (2021) Taxonomic profiling and functional characterization of the healthy human oral bacterial microbiome from the north Indian urban sub-population. Arch Microbiol., 203(3):927-939. DOI: 10.1007/s00203-020-02084-7
7. Singh, R. and Dubey, A. K. (2020) Isolation and characterization of a new endophytic actinobacterium Streptomyces californicus strain ADR1 as a promising source of anti-bacterial, anti-biofilm and antioxidant metabolites. Microorganisms 8(6):929, DOI: 10.3390/microorganisms8060929
8. Singh, R. and Dubey, A. K. (2020) Differential synthesis of secondary metabolites by Streptomyces chrestomyceticus strain ADP4 in response to modulation in nitrogen source and its anti-Candida activity. Proceedings 2020, 66, 5; doi:10.3390/proceedings2020066005
9. Chaubey, A. and Dubey, A. K. (2020) Chemistry and antioxidant potential of phytoconstituents from Aegle marmelos fruit-shell. Current Drug Metabolism, 21(7): 525-533. DOI: 10.2174/13892002-21666200711161056
10. Singla, R. K. and Dubey, A. K. (2019) Molecules and Metabolites from Natural Products as Inhibitors of Biofilm in Candida spp. pathogens. Curr. Topics Med. Chem.19 (28), 2567-2578.
11. Dubey, A. K. and Singla, R. K. (2019) Perspective on anti-Candida drug development Curr. Topics Med. Chem.19 (26), 2375-2376
12. Singla, R. K., Dubey, A. K. et. al. (2019) Natural Polyphenols: Chemical Classification, Definition of Classes, Subcategories and Structures. J. AOAC Intl., 102(5):1397-1400. doi: 10.5740/jaoacint.19-0133
13. Lingana, P., Anastasi, G., Marino, Piccione, S., Singla, R.K., Dubey, A. K. et. al., (2019) Phenolic Substances in Foods: Health Effects as Anti-Inflammation Agents and Antimicrobial Properties. J. AOAC Intl., 102(5):1378-1387. doi: 10.5740/jaoacint.19-0131
14. Singla, R. and Dubey, A. K. (2019) Phytochemical Profiling, GC-MS Analysis and α-Amylase Inhibitory Potential of Ethanolic Extract of Cocos nucifera Linn. Endocarp. Endocrine, Metabolic & Immune Disorders - Drug Targets, 19 (4), 419 – 442.
15. Srivastava, V., Singla, R. and Dubey, A. K. (2018) Inhibition of Biofilm and Virulence Factors of Candida albicans by Partially Purified Secondary Metabolites of Streptomyces chrestomyceticus Strain ADP4. Curr. Topics Med. Chem. 18, 925-945.
16. Singla, R. K., Ali, M, Kamal, M. A. and Dubey, A. K. (2018) Isolation and Characterization of Nuciferoic Acid, a Novel Keto Fatty Acid with Hyaluronidase Inhibitory Activity from Cocos nucifera Linn. Endocarp. Curr. Topics Med. Chem. 18, 2367 - 2378.
17. Srivastava, V., Singla, R. and Dubey, A. K. (2018) Emerging virulence, drug resistance and future anti-fungal drugs for Candida pathogens. Curr. Topics Med. Chem. 18, 759-778.
18. Singh R. and Dubey, A. K. (2018) Diversity and applications of endophytic actinobacteria of plants in Special and Other Ecological Niches. Front. Microbiol. 9:1767. doi: 10.3389/fmicb.2018.01767
19. Tiwary, M. and Dubey, A. K. (2018). Characterization of biosurfactant produced by a novel strain of Pseudomonas aeruginosa, isolate ADMT1. J Surfactants Deterg., 21: 113-125.
20. Verma, D., Garg, P. K., Dubey, A. K. (2018). Insights into the human oral microbiome. Arch. Microbiol., 200: 525 -540. doi: 10.1007/s00203-018-1505-3.
21. Sharma, K., Chandra, S., and Dubey, A. K. (2018) Exploration of lower frequency EEG dynamics and cortical alpha asymmetry in long-term rajyoga meditators. Intl. J. Yoga, 11(1): 30-36.
22. Sharma, K., Trivedi, R., Chandra, S., Kaur, P., Kumar, P., Singh, K., Dubey, A. K. and Khushu, S. (2018). Enhanced White Matter Integrity in Corpus Callosum of Long-Term Brahmakumaris Rajayoga Meditators. Brain Connectivity, 8(1). https://doi.org/10.1089/brain.2017.0524
23. Srivastava, V., Singla, R. K. and Dubey, A. K. (2017) Inhibition of Secretory Aspartyl Protease of Candida albicans by metabolites of Streptomyces chrestomyceticus strain ADP4. MOL2NET 2017, International Conference on Multidisciplinary Sciences, 3rd edition, 4969 (doi: 10.3390/mol2net-03-04969).
24. Singla, R. K., Scotti, L. and Dubey, A. K. (2017) In Silico Studies Revealed Multiple Neurological Targets for the Antidepressant Molecule Ursolic Acid. Curr. Neuropharmacol., 15(8): 1100-1106.
25. Srivastava, V. and Dubey, A. K. (2016) Anti-biofilm activity of the metabolites of Streptomyces chrestomyceticus strain ADP4 against Candida albicans. J. Biosci. Bioengg., 122 (4), 434 – 440.
26. Singla, R. K., Singh, R. and Dubey, A. K. (2016) Important aspect of post-prandial antidiabetic drug, Acarbose. Curr. Top. Med. Chem., 16, 2625-2633.
27. Tewari, M. and Dubey, A. K. (2016) Cypermethrin bioremediation in presence of heavy metals by a novel heavy metal tolerant strain, Bacillus sp. AKD1. Intl. Biodeterior., Biodegrad., 108, 42-47.
28. Singh, A., Meena, M., Kumar, D., Dubey, A. K. and Hassan, Md. I. (2015) Structural and functional analysis of various globulin proteins from soy seed. Critical Rev. Food Sci. Nut., 55: 1491–1502
29. Singh R. and Dubey AK (2015). Endophytic actinomycetes as emerging source for therapeutic compounds. Indo Global J Pharm. Sci., 5, 106–116.
30. Singla, R., Dubey, H. and Dubey, A. K. (2014) Therapeutic spectrum of bacterial metabolites. Indo Global J Pharm. Sci., 4(2), 52-64.
31. Singh, A., Thakur, P. K., Meena, M., Kumar, D., Bhatnagar, S., Dubey, A. K. and Hassan, Md. I. (2014) Interaction between basic 7S globulin and leginsulin in soybean [Glycine max]: Astructural insight. Lett. Drug Des. Discov. 11(2), 231-239.
32. Jaydev, M. S. K., Ralhan, R., Sen, S., Narayanasamy, K. and Dubey, A. K. (2012) Molecular mechanism of antiproliferative action of Phyllanthus emblica in HeLa cells. In: Kaushik, B. D. (ed.) Biotechnological interventions for the benefits of mankind, AEC Agra, India, pp 6-20.
33. Dubey, A. K., Bharadwaj, P. R., Varghese, J. N. and Macreadie, I. G. (2009) Alzheimer’s amyloid beta rescues yeast from hydroxide toxicity J. Alzheimers Dis. 18, 31-33.
34. Bharadwaj, P., Dubey, A. K., Masters, C., Martins, R., and Macreadie, I. G. (2009) Aβ aggregation and its implication in Alzhiemer’s disease. J. Cell. Mol. Medicine 13, 412-421.
35. Pathak, R., Bhatnagar, S. and Dubey, A. K. (2008). Mechanisms underlying the opposing effects of P2Y receptors on the cell cycle. J. Recept. Signal Transduct. 28, 505-529.
36. Bhatnagar, S. and Dubey, A. K. (2008) Bioinformatics. In: Microbiology e-Book, National Digital Science Library, http://hdl.handle.net/123456789/633.
37. Jaydev, M. S. K., Bhatnagar, S. and Dubey, A. K. (2007) Emerging infectious diseases in humans. In: Chauhan, A. K., Kharakwal, H. and Varma, A. K. (eds) Microbes for Human Life, IK International Publishers, New Delhi, India, pp 149-193.
38. Bhatnagar, S., Srivastava, D., Jayadev, M. S. K. and Dubey, A. K. (2006) Molecular variants and derivatives of insulin for improved glycemic control in diabetes. Prog. Biophys. Mol. Biol. 91, 199 – 228.
39. Jaydev, M. S. K., Bhatnagar, S. and Dubey, A. K. (2005) Genomics in diversity and pathogenicity of Flaviviruses. In: Satyanarayana, T. and Johri, B. N. (eds) Microbial Diversity: Current perspectives and potential applications, IK International Publishers, New Delhi, India, pp 1075-1096.
40. Jayadev, M. S. K. and Dubey, A. K. (2005) Molecular mechanisms in the symbiotic association between plant and arbuscular mycorrhizae. In: Podila, G. K. and Varma, A. (eds) Basic research and applications of mycorrhizae, IK International Publishers, New Delhi, India, pp 57-65.
41. Dubey, A. K. (2002) Resolution of a complex ionic mixture of an apparently homogenous protein preparation by preparative electrophoresis. Ind.J. Biochem. Biophys. 39, 279-283.
42. Bhattacharya, S. K. and Dubey, A. K. (2002) The N-terminus of m5C – DNA methyltransferase MspI is involved in its topoisomerase activity. Eur. J. Biochem. 269, 2491-2497.
43. Bhattacharya, S. K. and Dubey, A. K. (2002) Transient DNA binding by a proteolytic peptide from m5C-DNA methyltransferase MspI. J. Biochem. Mol. Biol. Biophys. 6, 357-364.
44. Bhattacharya, S. K. and Dubey, A. K. (2000) Amino acid residues involved in catalysis and DNA binding by cytosine DNA methyltransferase MspI. J. Biochem. Mol. Biol. Biophys. 4, 109-120.
45. Bhattacharya, S. K. and Dubey, A. K. (1999) Kinetic mechanism of cytosine DNA methyltransferase MspI. J. Biol. Chem. 274, 14743-14749.
46. Dubey, A. K. and Bhattacharya, S. K. (1997) Angle and locus of the bend induced by MspI DNA methyltransferase in sequence specific complex with DNA. Nucl. Acids Res. 25, 2025-2029.
47. Bhattacharya, S. K. and Dubey, A. K. (1997) High level expression of heterologous gene in Escherichia coli in response to carbon-nitrogen source and C/N ratio in batch bioreactor. Biotechnol. Prog. 13, 151-155.
48. Bhattacharya, S. K. and Dubey, A. K. (1997) Effects of dissolved oxygen and oxygen mass transfer on over-expression of target gene in E. coli. Enz. Microbial Technol. 20, 355-360.
49. Bhattacharya, S. K. and Dubey, A. K. (1996). Expression parameters for target gene cloned in Escherichia coli in response to phosphate supply. Biotechnol. Lett., 18, 1145-1148.
50. Dubey, A. K.; Mukhopadhyay, S. N. and Thukral, R. (1995). Recovery, purification and variables of activity of native BamHI. Indian Chem. Engr., 37, 169-175.
51. Bhattacharya, S. K. and Dubey, A. K. (1995). Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene. Biotechnol. Lett., 17, 1155-1160.
52. Bhattacharya, S. K. and Dubey, A. K. (1994). Preparation and properties of restriction endonuclease SphI coupled to nylon fabric filter. Biotechnol. Appl. Biochem., 20, 141-146.
53. Dubey, A. K.; Mollet, B. and Roberts, R. J. (1992). Purification and characterization of the MspI methyltransferase cloned and overexpressed in E. coli. Nucl. Acids Res., 20, 1579-1585.
54. Dubey, A. K. and Roberts, R. J. (1992). Sequence specific DNA binding by MspI DNA methyltransferase. Nucl. Acids Res., 20, 3167-3173.
55. Dubey, A. K.; Mukhopadhyay, S. N.; Bisaria, V. S. and Ghose, T. K. (1991). Upstream bio-processing for BamHI production: effect of cell cultivation variables. Ind. Chem. Engr., 33, 16 - 21.
56. Dubey, A. K.; Bisaria, V. S.; Mukhopadhyay, S. N. and Ghose, T. K. (1989). Stabilization of restriction endonuclease BamHI by cross-linking reagents. Biotechnol. Bioeng., 33, 1311-1316.
57. Dubey, A. K. and Johri, B. N. (1987). Xylanolytic activity of thermophilic Sporotrichum sp. and Myceliophthora thermophilum. Proc. Indian Acad. Sci. (Plant Sciences), 97, 247-255.
58. Dubey, A. K.; Mukhopadhyay, S. N.; Bisaria, V. S. and Ghose, T. K. (1987). Sources, production and purification of restriction enzymes. Process Biochem., 22, 25-34.

16 rRNA gene Sequences, submitted to NCBI GeneBank Database

1. Srivastava SR, Tiwary M, Dubey AK (2013) Bacillus sp. AKD1 16S ribosomal RNA gene sequence, partial sequence, KC212686.1
2. Kaushal I, Srivastava V, Chowdhary A, Dubey AK (2015) Streptomyces puniceus strain ADAT1 16S ribosomal RNA gene, partial sequence, KT781123.1
3. Kaushal I, Srivastava V, Chowdhary A, Dubey AK (2015) Streptomyces rochei strain ADLK1 16S ribosomal RNA gene, partial sequence, KT781124.1
4. Singh R, Dubey AK (2015) Streptomyces californicus ADR1 16S ribosomal RNA gene sequence, partial sequence, KU299789.1
5. Srivastava V, Kaushal I, Chowdhary A, Dubey AK (2015) Nocardiopsis dassonvillei strain ADVJ1 16S ribosomal RNA gene, partial sequence, KT781122.1
6. Srivastava V, Kaushal I, Chowdhary A, Dubey AK (2015) Streptomyces variabilis strain ADAR1 16S ribosomal RNA gene, partial sequence, KT781121.1
7. Srivastava V, Kaushal I, Chowdhary A, Dubey AK (2015) Streptomyces chrestomyceticus strain ADP4 16S ribosomal RNA gene, partial sequence, KT781120.1
8. Tiwary M, Dubey AK (2015) Pseudomonas aeruginosa ADMT1 16S ribosomal RNA gene sequence, partial sequence, KP036461.1
9. Tiwary M, Dubey AK (2015) Streptomyces chilikensis ADMT10 16S ribosomal RNA gene sequence, partial sequence, KT454969.1
10. Tiwary M, Dubey AK (2015) Streptomyces erythrogriseus ADMT11 16S ribosomal RNA gene sequence, partial sequence, KT454970.1
11. Tiwary M, Dubey AK (2015) Streptomyces rameus ADMT3 16S ribosomal RNA gene sequence, partial sequence, KT454968.1
12. Tiwary M, Dubey AK (2015) Streptomyces sp. ADMT2 16S ribosomal RNA gene sequence, partial sequence, KT454967.1
13. Tiwary M, Dubey AK (2016) Micromomspora sp. ADMT18 16S ribosomal RNA gene sequence, partial sequence, KU976154.1
14. Tiwary M, Dubey AK (2016) Micromomspora sp. ADMT19 16S ribosomal RNA gene sequence, partial sequence, KU976155.1
15. Tiwary M, Dubey AK (2016) Saccharomonospora sp. ADMT12 16S ribosomal RNA gene sequence, partial sequence, KU976152.1
16. Tiwary M, Dubey AK (2016) Streptomyces sp. ADMT14 16S ribosomal RNA gene sequence, partial sequence, KU976153.1

17. Tiwary M, Dubey AK (2016) Streptomyces sp. ADMT8 16S ribosomal RNA gene sequence, partial sequence, KY353349
18. Tiwary M, Dubey AK (2016) Streptomyces sp. ADMT9 16S ribosomal RNA gene sequence, partial sequence, KU976151.1
19. Singh, R., Dubey, A. K. (2015). Streptomyces californicus strain ADR1 16S ribosomal RNA gene, partial sequence. GenBank: KU299789.1
20. Singh, R., Dubey, A. K. (2018). Streptomyces deccanensis strain ADR2 16S ribosomal RNA gene, partial sequence. GenBank: MH635328.1.
21. Singh, R., Dubey, A. K. (2018). Streptomyces europaeiscabiei strain ADR3 16S ribosomal RNA gene, partial sequence. GenBank: MH635329.1.
22. Singh, R., Dubey, A. K. (2018). Pseudonocardia carboxydivorans strain ADR4 16S ribosomal RNA gene, partial sequence. GenBank: MH635330.1
23. Singh, R., Dubey, A. K. (2018). Micromonospora haikouensis strain ADR5 16S ribosomal RNA gene, partial sequence. GenBank: MH635331.1
24. Singh, R., Dubey, A. K. (2018). Micromonospora harpali strain ADR6 16S ribosomal RNA gene, partial sequence. GenBank: MH635332.1
25. Singh, R., Dubey, A. K. (2018). Pseudonocardia carboxydivorans strain ADR7 16S ribosomal RNA gene, partial sequence. GenBank: MH635333.1
26. Singh, R., Dubey, A. K. (2018). Micromonospora oryzae strain ADR8 16S ribosomal RNA gene, partial sequence. GenBank: MH635334.1
27. Singh, R., Dubey, A. K. (2018). Micromonospora harpali strain ADR9 16S ribosomal RNA gene, partial sequence. GenBank: MH635335.1